Which of the following restriction enzymes produces blu
Which of the following restriction enzymes produces blunt ends?
A.
Xho I
B.
Hind III
C.
Sal I
D.
EcoR V
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Right Answer is: D
SOLUTION
Restriction enzymes are proteins that cut DNA at specific recognition sites. Two types of restriction enzymes exist that differ in the way they cut the target DNA:
Blunt end cutters - These enzymes cut both strands of the target DNA at the same spot creating blunt ends.
Sticky end cutters - These enzymes cut both strands of the target DNA at different spots creating 3'- or 5'-overhangs of 1 to 4 nucleotides (so-called sticky ends).
The restriction enzyme that produces blunt ends is - EcoRV is a type II restriction endonuclease isolated from certain strains of Escherichia coli. It has the alternative name Eco32I. It creates blunt ends.